长柔毛野豌豆叶绿体基因组特征与系统发育OA

Vicia villosa belongs to the Vicia genus of the Fabaceae family and is an annual herb that prefers to grow in moist and organic-rich habitats.Although the chloroplast genome study of Fabaceae has gradually progressed in recent years,the chloroplast genome of Vicia villosa,a member of the IRLC(inverted repeat lacking clade)taxon,has not yet been assembled.This study is based on NCBI V.villosa whole genome resequencing data and assembled and analyzed its chloroplast genome.These results showed that the full length of the V.villosa chloroplast genome is 124228 bp,with 34.63%GC content,and it lacks the inverted repeat IR region,does not have the typical tetrad structure,and belongs to the species lacking in inverted repeat sequence.This chloroplast genome has 110 genes,including 76 CDS,4 rRNA genes and 30 tRNA genes.Codon analysis showed a preference for A/T bases for its chloroplast genome codons.Repeat sequence analyses detected 65 SSRs,and these loci were mostly in the form of mononucleotide repeats,and in the case of long repeat sequences mainly forward repeats were dominant.In addition,the overall variation among their chloroplast genomes was small compared to their close relatives,but significant differences existed in the ycf4,rps11,rpoA,trnL-UAG～trnF-GAA,trnI-CAU,trnL-CAA,trnH-GUG～trnN-GUU,and ndhB regions.Phylogenetic analyses showed that Vicia villosa and Vicia cracca are relatively close to each other,indicating that they may share a common ancestor during the evolutionary process.This study provides some basic data support for the genetic diversity analysis of Vicia and the development and utilization of wild resources.