两系杂交稻组合芽期和苗期耐盐性鉴定及其产量协同分析OA

In this study,54 two-line hybrid rice varieties with varying degrees of salt tolerance were used as experimental materials.Rice plants were treated with salt at different concentrations during the germination and seedling stages,and indicators including the germination potential,germination rate,shoot length,root length,root number,shoot fresh weight,and whole-plant fresh weight were measured.The mean value of the salt injury rate for each indicator was used as the basis for evaluating salt tolerance.The results showed that with the increase of salt concentrations,the inhibitory effects on seed germination and seedling growth were significantly enhanced.The salt injury rates of various traits exhibited similar trends across different salt concentrations.Through salt stress treatment and analysis,five salt-tolerant combinations,namely Shuangliangyou 521,Shuangliangyou 1143,Shuangliangyou 182,Shuangliangyou 138,and Shuangliangyou 132 were screened out.Notably,Shuangliangyou 521,Shuangliangyou 182,Shuangliangyou 138,and Shuangliangyou 132 demonstrated higher per-plant yields in non-saline-alkali fields,revealing a favorable synergy between salt tolerance and high yield.Among all the tested combinations,Shuangliangyou 521 exhibited the best salt tolerance,with the lowest mean salt injury rate(22.80%)at the seedling stage.The results of multi-index evaluation showed that this combination demonstrated extremely strong salt tolerance regarding the salt injury rate of whole-plant fresh weight under 0.8%NaCl treatment and the salt injury rate at the seedling stage under 1.2%NaCl treatment,which were 2.29%and 20.00%,respectively.It exhibited strong salt tolerance based on the salt injury rates of shoot fresh weight under 0.8%NaCl treatment and whole-plant fresh weight,root number,and shoot fresh weight under 1.0%NaCl treatment,which were 25.10%,27.73%,31.30%,and 38.89%,respectively.The salt injury rates of root length and shoot length under 1.0%NaCl treatment at 52.76%and 58.19%,respectively,indicated moderate salt tolerance.Meanwhile,the yield per plant(49.84 g)of this combination ranked second among all tested combinations.This study provides reference for the promotion and layout of hybrid rice combinations of Shuangliangyou series in both non-saline-alkali and saline-alkali fields.