夹脊穴埋线联合跑台训练对脊髓损伤大鼠脊髓神经修复及运动功能的影响OA

Objective:To investigate the effects and underlying mechanisms of Jiaji acupoints catgut embedding(AE)combined with treadmill training(TM)on spinal cord neural repair and motor function in rats with spinal cord injury(SCI).Methods:The SCI model was established using the modified Allen's method.The successfully modeled rats were randomly divided into the SCI group,the AE group,the TM group,and combined group(AE+TM)group,with an additional sham operation(Sham)group,with 10 rats in each group.Rats in the AE,TM,and AE+TM groups received respective interventions for 4 continuous weeks.Behavioral changes were assessed using the Basso-Beattie-Bresnahan(BBB)locomotor rating scale,inclined plane test,and footprint analysis.Spinal cord nerve conduction velocity was measured using a multichannel electrophysiological recorder.Histopathological changes and neuronal survival in spinal cord tissue were observed using HE staining and Nissl staining.Levels of IL-1β,IL-6,and TNF-α in spinal cord tissue were determined by ELISA.Western blot analysis was used to detect the protein expressions of related to the brain-derived neurotrophic factor(BDNF)/tropomyosin receptor kinase B(TrkB)signaling pathway in spinal cord tissue.Results:Compared with the Sham group,rats in the SCI group showed significantly decreased BBB scores,inclined plane angles,and hindlimb stride length and stride width(P＜0.05);the levels of IL-1 β,IL-6,and TNF-α in spinal cord tissue were significantly increased(P＜0.05),while nerve conduction velocity,neuronal survival,BDNF protein expression,and the p-TrkB/TrkB protein ratio were significantly decreased(P＜0.05).Compared with the SCI group,rats in the AE,TM,and AE+TM groups exhibited significantly increased BBB scores,inclined plane angles,and hindlimb stride length and stride width(P＜0.05);the levels of IL-1β,IL-6,and TNF-α in spinal cord tissue were significantly decreased(P＜0.05),whereas nerve conduction velocity,neuronal survival,BDNF protein expression,and the p-TrkB/TrkB protein ratio were significantly increased(P＜0.05).The AE+TM group showed significantly greater improvements in spinal cord neural repair and motor function than the AE group and the TM group(P＜0.05).Conclusion:AE combined with TM promotes spinal cord neural repair and improves motor function in rats with SCI,and its mechanism may be related to activation of the BDNF/TrkB signaling pathway.