巴戟天种质复壮组织培养快繁技术体系研究OA

In order to establish a tissue culture rapid propagation technology system for the rejuve-nation of the medicinal plant Morinda officinalis,using the young shoot tips of the main cultivated variety Heisuizai in Zhaoqing as the explants,the effects of MS and N6 culture media and different concentrations of 6-BA and IBA combinations on their induction,differentiation,proliferation and rooting were studied.The most suitable culture media at each developmental stage were screened.The results showed that the MS medium had the best induction effect.The initial generation bud induction medium was MS+IBA 0.10 mg/L+6-BA 1.50 mg/L.The secondary generation proliferation medium was MS+6-BA 0.5-1.00 mg/L+IBA 0.10 mg/L.The survival rate of adventitious buds was 95.56％-98.23％,and the number oftillers was 6.97-7.66.The vigorous seedling culture medium was MS+6-BA 0.30 mg/L+IBA 0.10 mg/L.The rooting culture medium was MS+1.00 g/L activated carbon+1.00 mg/L IBA.The rooting rate was 95.75％,and the rooting coefficient was 11.17.After the o-riginal bottle of the tissue culture seedlings was acclimatized for 15 days,which were transplanted to peat soil or peat soil+river sand substrate.The survival rate reached 98.33％after 70 days and the growth was vigorous.