柔毛淫羊藿种子休眠破除过程中胚发育动态及萌发促进技术研究OA

To solve the problems of depletion of wild resources of Epimedium pubescens and the deep dormancy of its seeds,which hindered artificial cultivation,various treatments such as low-tempera-ture stratification,variable-temperature stratification,200 mg/L gibberellin(GA3),10 mg/L fluri-done(FL)and their combinations were used to break the dormancy.The dynamic changes of seed em-bryo length,width and germination rate within 90 days were systematically observed,and the germi-nation rate and germination potential were statistically analyzed.The dormancy characteristics of Epimedium pubescens seeds and the breaking dormancy methods were systematically analyzed.The results showed that the seeds of Epimedium pubescens were of a morphological-physiological com-bined dormancy type.The dynamic development of the embryo showed that the 200 mg/L GA3 treat-ment could effectively promote the morphological ripening of the embryo,with an embryo rate of 92.66％;although the 10 mg/L FL treatment promoted the transformation of the seed embryo from spherical to heart-shaped in the early stage(30 days),of which promoting effect on the embryo mor-phology was weaker than that of the GA3 treatment,but it had a significant effect in breaking physio-logical dormancy and promoting germination,with the highest germination rate of 69.22％.The GA3 and FL combined treatment did not show a synergistic effect.The breaking dormancy of Epimedium pubescens seeds existed through 2 relatively independent pathways:morphological ripening and physi-ological unlocking.Among them,GA3 dominated the embryo morphology formation,while FL effi-ciently unlocked the physiological dormancy.Using 10 mg/L FL immersion for 6 hours followed by 4℃ dark stratification for 90 days was the preferred scheme for breaking the seed dormancy and achieving efficient germination.