不同萝卜品种种子萌发期耐低温性综合评价及关键指标筛选OA

In order to comprehensively evaluate the cold tolerance of different varieties of Raphanus sativus seeds during germination,and to select the key indicators for cold tolerance,43 different Raphanus sativus varieties were used as experimental materials.Through setting 2 temperature treat-ments of 15℃ and 25℃,9 indicators such as germination rate,germination potential,germination index,vitality index,root length,shoot length,fresh weight of root,fresh weight of shoot,and root-shoot ratio were selected for measurement.Correlation analysis,principal component analysis,membership function value analysis,cluster analysis and grey correlation degree analysis were con-ducted on the relative values of these indicators to conduct comprehensive evaluation and select the key indicators for cold tolerance.The results showed that principal component analysis transformed 9 individual indicators into 3 factors,with a cumulative contribution rate of 85.700％.The L05(Fumei No.3)had the largest D value(0.79),indicating the strongest cold tolerance.While L11(Junchuan youyue)had the smallest D value(0.05),indicating the poorest cold tolerance.Cluster analysis classi-fied the Raphanus sativus varieties into 5 categories,including 3 highly cold-tolerant varieties,13 cold-tolerant varieties,19 moderately cold-tolerant varieties,5 sensitive varieties,and 3 highly sensi-tive varieties.Through grey correlation degree analysis,it was found that each relative trait indicator had a highly significant positive correlation with the D value,and the relative values of germination potential,vitality index,germination rate,germination index,shoot length and root length were selected as the key indicators for cold tolerance during the Raphanus sativus seed germination period.L05(Fumei No.3),L13(Lüfushi No.7),and L30(Zhaofu)were selected as cold-tolerant varieties.