盐酸度洛西汀抗白念珠菌活性初探OA

Objective To systematically evaluate the effects of duloxetine hydrochloride on Candida albicans through in vitro experiments.Methods The minimum inhibitory concentration(MIC)and minimum fungicidal concentration(MFC)of duloxetine hydrochloride against the growth of C.albicans were detected by micro-broth dilution method;the time-fungicidal curve was used to examine the time-fungicidal trend of duloxetine hydrochloride against C.albicans.The effects of different concentrations of duloxetine hydrochloride on the morphological transformation of C.albicans were observed by inverted microscope;and the effects on the biofilm metabolic activity and biofilm formation of C.albicans were evaluated by the methyl tetrazolium salt(XTT)method and crystal violet staining.Results The MIC of C.albicans was 15.625 µg/mL,and the MFC was 31.25 µg/mL;31.25 µg/mL of duloxetine hydrochloride effectively killed C.albicans at 9 h;31.25 µg/mL and 15.625 µg/mL of duloxetine hydrochloride prevented the conversion of C.albicans from yeast to mycelial phase and allowed C.albicans to remain in yeast phase.In addition,duloxetine hydrochloride inhibited C.albicans biofilm metabolic activity and biofilm volume as well,and its inhibitory effect was concentration-dependent.Conclusion Duloxetine hydrochloride has significant antifungal activity against C.albicans in vitro.