植物乳植杆菌与枯草芽孢杆菌对脆秆水稻秸秆青贮发酵质量的影响OA

[Objective]This study aimed to explore the effects of adding Lactiplantibacillus plantarum(LP)and Bacillus subtilis(BS)during the ensiling of fresh brittle culm rice straw,in order to develop its potential as a forage resource,improve straw utilization as feed,and alleviate the shortage of feed resources in animal husbandry.[Method]A two-factor experimental design was adopted,factor A was fermentation duration(15,30,and 60 d),and factor B was inoculant treatment:No inoculant(CK),adding 106 CFU/g BS(BSLP0),106 CFU/g LP(BS0LP),and 106 CFU/g LP+BS(BSLP).A total of 12 treatment groups were set up.After thorough mixing of inoculants with brittle culm rice straw silage,samples were taken and wrapped,followed by storage at room temperature in the dark.After ensiling,samples were unpacked and analyzed for fermentation quality and nutrient quality indicators.[Result]A significant interaction was found between fermentation duration and inoculant treatment(P<0.05),indicating that different inoculants performed differently at various fermentation stages.Fermentation duration and inoculant treatment both significantly affected fermentation indicators of brittle culm rice straw silage(P<0.05).Compared with CK and single-inoculant groups,the combined LP+BS inoculant effectively promoted lactic acid(LA)production,and significantly reduced pH(P<0.05).Fermentation duration and inoculant treatment also significantly influenced the contents of dry matter(DM),crude protein(CP),and crude ash(Ash)of brittle culm rice straw silage(P<0.05).The contents of CP,water soluble carbohydrates(WSC),and ether extract(EE)were the highest at fermentation 60 d.The combined inoculant showed superior effects in preserving nutrient quality compared with single-inoculant,with CP content significantly higher than that in BS-alone group(P<0.05),and higher than that in LP-alone group.Fermentation duration and inoculant treatment significantly affected the acid washed lignin(ADL)of brittle culm rice straw silage(P<0.05),compared with CK group,the combined LP+BS inoculant showed increased ADL content,while no significant effects were observed on the contents of neutral detergent fiber(NDF)and acid detergent fiber(ADF)(P>0.05).[Conclusion]Brittle culm rice straw was suitable for ensiling.The combined application of LP and BS yielded better ensiling results than single inoculant.Ensiling for 60 d with the combined inoculant most effectively improved the fermentation quality and nutrient preservation of brittle culm rice straw silage.