基于层次分析法-熵权法的低等级生物安全实验室风险评估体系的构建OA

Objective The rapid development of biotechnology has highlighted the importance of biosafety in the field of public health;however,the large number and wide distribution of low-level biosafety laboratories present potential risks that urgently need effective identification,control,and prevention.Methods This study targeted frontline biosafety laboratory personnel from hospitals,community diagnostic centers,and health-administration departments.Two rounds of anonymous expert consultations were conducted using the Delphi method,followed by a combination of an analytic hierarchy process and entropy weight method for integrated weighting,ultimately forming a risk assessment indicator system for low-level biosafety laboratories combining subjective and objective evaluations.Results Thirty experts participated in the first round and 35 in the second.Two rounds of anonymous consultation and feedback revisions produced a systematic risk-assessment indicator system,including five primary indicators(A1～A5:personnel risk prevention and control,equipment safety management,sample risk management,laboratory system construction,laboratory environmental management),10 secondary indicators(B1-1～B5-1),and 30 tertiary indicators(C1-1-1～C5-1-3).The Kendall harmony coefficient was 0.23(P＜0.001),and the consistency ratios of the judgment matrices at all levels were less than 0.1,indicating good coordination of expert opinions and reasonable weight allocation.Conclusions A unified risk-assessment indicator system for low-level biosafety laboratories was constructed based on dimensions such as personnel,equipment,samples,regulations,and environment,with quantified indicator weights.This enriches research on standardized assessments in this field.The system is applicable to daily risk management of low-level biosafety laboratories,supervision and inspection by regulatory authorities,and related academic research.