连翘苷调节JAK2/STAT3信号通路对大鼠痔术后创面愈合及炎症反应的影响及作用机制OA

Objective To explore the effects of phillyrin(Phil)on wound healing and the inflammatory response after hemorrhoid surgery in rats by adjusting the Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods A rat model of hemorrhoids was established by acetic acid injection.Rats were divided randomly into Model,Phil-low-dose(Phil-L),Phil-high-dose(Phil-H),Phil-H+JAK2A activator coumermycin A1(CA1),and healthy control(HC)groups(n=8 rats per group).The wound area was measured using Vernier calipers,and the degree of perianal edema was observed and evaluated.The total blood cell count was used to detect the numbers of white blood cells,neutrophils,and lymphocytes.Serum levels of tumor necrosis factor(TNF)-α,interleukin(IL)-6,and IL-1β were detected by ELISA.Pathological changes in perianal tissues were observed by hematoxylin/eosin staining.JAK2 andSTAT3 mRNA in perianal tissues were detected by RT-qPCR and vascular endothelial growth factor(VEGF),matrix metalloproteinase(MMP)-9,and JAK2/STAT3 pathway-related proteins in perianal tissues were detected by immunoblotting.Results Compared with the Model group,Phil significantly reduced inflammatory cell infiltration in perianal tissue;decreased the wound area and perianal edema;reduced the white blood cell count,neutrophil percentage,JAK2,STAT3 mRNA and IL-1β,IL-6,TNF-α,phospho-JAK2,and p-STAT3 proteins;and increased the proportion of lymphocytes and VEGF and MMP-9 proteins(all P＜0.05).The differences between the Phil-L and Phil-H groups were also significant(all P＜0.05).All quantitative indicators showed opposite expression in the Phil-H+CA1 and Phil-H groups(all P＜0.05).Conclusions Phil can inhibit the JAK2/STAT3 signaling pathway,suppress the inflammatory response after hemorrhoid surgery in rats,and promote wound healing.