二倍体马铃薯种质资源筛选与评价OA

Diploid potatoes are important germplasm resources.Screening and evaluating diploid potato germplasm resources can provide valuable materials for the development of superior new potato germplasm.To identify for self-compatible and high-yielding diploid potatoes,this study evaluated 68 potato germplasm resources(including 57 diploids and 11 tetraploids)for chromosome ploidy,self-compatibility,and field agronomic traits.Regarding ploidy identification,by comparing three methods-leaf length-width ratio,guard cell chloroplast counting,and flow cytometry-chloroplast counting was identified as a simple and effective method for determining potato ploidy.For self-compatibility identification,6 self-compatible accessions were identified using the Sli gene molecular marker,including 1 tetraploid accession and 5 diploid accessions.Through field self-pollination experiments,33 diploid self-compatible accessions were identified,4 of which contained the Sli gene molecular markers.The field agronomic evaluation results showed that the diploid accession G18-27-27 exhibited the highest average single-plant yield of 1.23 kg,and was self-compatible;R8209 produced the highest number of tubers per plant with 25.25 tubers;among the accessions carrying Sli gene marker,YN20S225-1 had the highest single-plant yield of 0.86 kg,and YN20S145-1 produced the highest number of tubers per plant,with 24.92 tubers.The evaluation methods screened and the potato germplasm resources evaluated in this study provide a technical reference and material basis for the improvement of diploid potato germplasm.