结球甘蓝游离小孢子培养技术研究进展OA

Isolated microspore culture serves as a highly efficient breeding tool capable of rapidly generating homozygous doubled haploid(DH)materials,significantly shortening the breeding cycle.This technology holds substantial importance for improving the breeding efficiency of Brassica vegetables.This paper reviews the research progress of isolated microspore culture technology in China,specifically focusing on its applications in disease-resistant breeding,molecular marker-assisted breeding,and genetic resource innovation in Brassica oleracea L.It elaborates on the effects of key factors including donor genotype,selection of donor material at the optimal stage,heat shock treatment,medium optimization,plant growth regulators,and additives on embryoid induction and plant regeneration.Furthermore,the study proposes future prospects for further enhancing the efficiency and precision of Brassica oleracea L.through the optimization of the microspore culture technical system.