睡眠片段化对高脂喂养小鼠心脏重塑的作用研究OA

Background Sleep fragmentation(SF)is one of the hallmarks of sleep disorders,and the mechanism in cardiac remodeling is still unclear.Objective To observe the effect of SF on cardiac remodeling in mice with high-fat diet.Methods The research period was from August 2023 to June 2024.Twenty-one 8-week-old male C57BL/6J mice were randomly divided into the high-fat sleep fragmentation group(SF group,n=11)and the high-fat control group(Ctrl group,n=10).They were placed in the sleep deprivation apparatus and the normal cage respectively.Both groups were given 45%high-fat feed,and the sleep fragmentation intervention lasted for 5 months.The degree of cardiac hypertrophy was evaluated by heart weight/body weight(HW/BW)and heart weight/tibia length(HW/TL).The changes in cardiac structure were evaluated by echocardiography.Myocardial HE staining,Masson staining,and WGA staining were used to observe the size of myocardial cells and collagen content.The heart tissues of mice were sequenced for transcriptomics and metabolomics and the mechanism in cardiac remodeling was analyzed.Results Compared with the Ctrl group,the heart weight of the SF group increased(P<0.05),HW/BW and HW/TL rose(P<0.05),left ventricular internal dimension diastole,left ventricular internal dimension in systole,left ventricular end-diastolic volume,left ventricular end-systolic volumes all increased(P<0.05),and the left ventricular posterior wall thickness during diastole decreased(P<0.05).HE staining showed that compared with the Ctrl group,the heart of the SF group was enlarged and the cardiomyocytes were enlarged;the statistical graph of Masson staining showed that compared with the Ctrl group,the myocardial fibrosis of the SF group increased(P<0.01);the statistical graph of WGA staining showed that compared with the Ctrl group,the cardiomyocytes of the SF group were enlarged(P<0.001).The transcriptomics results suggested that the TGF-β signaling pathway in the SF group was upregulated.Metabolomics suggested that the increase in cardiac remodeling in the SF group was related to the downregulation of glycerophospholipid metabolism pathways.Conclusion Sleep fragmentation can lead to cardiac enlargement,ventricular dilation,ventricular wall thinning,increased collagen content,and cardiac remodeling in mice with a high-fat diet.The mechanism may be related to up-regulation of TGF-β signaling pathway and down-regulation of glycerophospholipid metabolism.