基于CRISPR/Cas9基因编辑技术研究AT1同源基因对小麦耐盐碱性的影响OA

alkaline tolerance 1(AT1),a gene cloned from sorghum,is involved in alkaline tolerance.In wheat,AT1 homologs are located on chromosomes 4A,7A and 7D.To explore the role of AT1 gene in improving salt-alkali tolerance of wheat,CRISPR/Cas9 gene editing technology was used to construct AT1 gene knockout lines with the wheat variety'Fielder'as the background.A total of 8 gene-edited lines were obtained,among which AT1 homologous genes on chromosomes 4A,7A and 7D were all edited in 6 lines.Plants were subjected to neutral salt stress(100 mmol·L-1),alkaline salt stress(60,125 mmol·L-1)and combined saline-alkali stress(135 mmol·L-1)and the seedling length,fresh weight and dry weight of wild-type plants and mutants were determined and analyzed.The results showed that there were differences in the significance levels of AT1 gene-edited materials under different stress treatments:the significance level was the lowest under salt stress,followed by that under saline-alkali stress,and the highest under alkali stress.Above results provided insights into the potential application of AT1 homologs in enhancing saline-alkali tolerance in wheat.