基于HS-GC-IMS法分析不同基原川贝母药材挥发性成分及其标志物OA

Objective To investigate the composition and differential characteristics of volatile organic compounds(VOCs)in Fritillariae Cirrhosae Bulbus(FCB)from six legally authorized origins,and provide a reference for origin identification and quality evaluation of FCB.Methods Headspace-gas chromatography-ion mobility spectrometry(HS-GC-IMS)was employed to analyze the volatile components in dried bulbs of Fritillaria cirrhosa,F.unibracteata,F.przewalskii,F.delavayi,F.taipaiensis and F.unibracteata var.Wabuensis.Qualitative matching was performed using the built-in NIST and IMS database of GC-IMS Library Search.Principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA),and hierarchical cluster analysis(HCA)were applied to evaluate the differences in volatile components among samples from different origins.Results A total of 90 volatile organic compounds were preliminarily identified,mainly including aldehydes,alcohols,ketones,esters,carboxylic acids,pyrazines,and sulfur-containing compounds.Among them,aldehydes,alcohols,and ketones were the major categories.PCA and OPLS-DA results indicated that FCB samples from the six origins could be well distinguished.Based on variable importance in projection(VIP)values,17 potential differential markers were screened out,including ethyl acetate,2-propanone,a-phellandrene,3-hydroxy-2-butanone,methyl acetate,2-propanethiol,2-isobutyl-3-methoxypyrazine,propyl acetate,3-methylbutanal,1-butanol,2-methyl-2-propenal,hexanal,3-methyl-l-pentanol,2-methylpropanoic acid,3-butenenitrile,benzaldehyde,and 2-octanone.Cluster heatmap analysis further demonstrated that the above differential compounds could reflect the volatile metabolic characteristics of FCB from different origins.Conclusion HS-GC-IMS combined with chemometrics can effectively characterize the differences in volatile organic compounds among FCB from different origins,providing technical reference for origin identification,quality evaluation,and characteristic component screening of FCB.