基于超分子传感阵列的胶类中药特征研究及应用OA

Objective To establish a method for differentiating gelatinous Chinese medicines(GCMs)based on supramolecular sensing technology.Methods An indicator displacement assay(IDA)strategy was employed where test sample solutions of GCMs served as analytes and macrocyclic molecule-dye host-guest pairs with specific fluorescence signals were used as sensing pairs to construct a sensor array.The fluorescence intensity changes resulting from the interaction between different GCMs and the sensing units were detected and combined with pattern recognition methods for differentiation.Results Through screening host-guest sensing units with selective fluorescence responses to GCMs from a supramolecular library consisting of cyclodextrins,cucurbiturils,calixarenes,and biphen[n]arenes hosts combined with dyes,a quaternary supramolecular sensor array was constructed including quaternary ammonium salt calix[5]arene(QAC5A)·eosin Y(EY)(0.4 μmol/L vs 0.5 μmol/L),quaternary ammonium modified azocalix[4]arene(QAAC4A)·EY(1 μmol/L vs 1 μmol/L),dodecyl quaternary ammonium salt azo calix[4]arene(QAAC4A12C)·EY(0.6 μmol/L vs 1 μmol/L),and terphen[3]arene(WTP3)·fluorescein(F1)(0.3 μmol/L vs 0.6 μmol/L).Principal component analysis(PCA)demonstrated that Ejiao(Colla Corii Asini),Xin'ejiao(Colla Corii Suilli),Biejiajiao(Colla Carapacis Trionychis),Lugujiao(Colla Ossis Cervi),Guijiajiao(Colla Carapacis et Plastri Testudinis)or Lujiajiao(Colla Cornus Cervi)could be significantly distinguished within a 95％confidence interval.Conclusion This study developed a quaternary supramolecular sensor array integrated with pattern recognition analysis which enables rapid discrimination of various GCMs.