排骨灵化学成分研究OA

Objective To investigate the chemical constituents of Fissistigma bracteolatum.Methods The 95％ethanol extract of F.bracteolatum was successively separated by solvent partitioning,macroporous resin,silica gel,MCI,Sephadex LH-20,ODS column chromatography,and semi-preparative HPLC to obtain individual compounds.Their structures were elucidated using modern spectroscopic techniques such as NMR and MS.An in vitro inflammatory model was established using lipopolysaccharide(LPS)-stimulated RAW264.7 macrophages to evaluate the anti-inflammatory activity of the isolated compounds.Results A total of 22 compounds were isolated from F.bracteolatum,comprising of 12 flavonoids,8 amides,1 lignan,and 1 lactone.These were determined as:5,7-dihydroxy-6,8-dimethoxyflavone(1),5-hydroxy-7,8-dimethoxyflavone(2),quercetin(3),rutin(4),5,7,8-trimethoxydihydroflavone(5),5,6,8-trimethoxy-7-hydroxydihydroflavone(6),6,7-dimethoxy-8-hydroxydihydroflavone(7),(2SS)-7-hydroxy-6,8-dimethoxydihydroflavone(8),dihydroquercetin(9),aromadendrin(10),4,2',6'-trihydro xy-3',4'-dimethoxy-dihydrochalcone(11),2',6'-dihydroxy-4'-methoxydihydrochalcone(12),N-trans-sinapoylmethoxytyramine(13),N-[2-(4-hydroxy-3-methoxyphenyl)ethyl]-3-phenyl-2-propenamide(14),(E)-3-(4-hydroxy-3-methoxyphenyl)-N-phenethyl-acrylamide(15),N-trans-feruloyltyramine(16),N-trans-p-coumaroyltyramine(17),N-ciss-feruloyltyramine(18),cis-N-feruloyl-3-O-methyldopamine(19),cannabisin D(20),3-caffeoyl-2-methyl-D-erythronic acid-1,4-lactone(21),and(-)-syringaresinol(22).Conclusion Compounds 1,7,9-11,13-15,17,19,and 21 were isolated from the genus Fissistigma for the first time.The anti-inflammatory activity results demonstrated that compounds 5,9,13,and 16 significantly inhibited the release of nitric oxide in LPS-induced RAW264.7 macrophages.