黄连外泌体联合沙门氏菌外膜囊泡缓解结肠炎及其作用机制研究OA

Objective To explore the ameliorative effect and mechanism of Coptis chinensis exosomes(CCEs)combined with Salmonella outer membrane vesicles(SOMVs)on dextran sulfate sodium(DSS)-induced colitis in mice.Methods CCEs and SOMVs were prepared and characterized.A mouse model of DSS-induced colitis was established,and the mice were treated with CCEs,SOMVs alone or their combination.The disease activity index(DAI)and pathological changes were e-valuated.Serum inflammatory factors,intestinal barrier proteins and oxidative stress indicators in colon tissues were detec-ted.Results Both CCEs and SOMVs exhibited typical vesicle structures.Compared with the model group and the monother-apy groups,the combined group achieved the optimal improvement on colitis symptoms and pathological lesions(P＜0.01).The levels of serum TNF-α,IL-6 and IL-1β were significantly reduced.The expressions of Claudin-1,Occludin and SOD in colon tissues were markedly upregulated,while the content of MDA was downregulated.Conclusion The combina-tion of CCEs and SOMVs can ameliorate colitis in mice,and the mechanism involves synergistic anti-inflammation,intesti-nal barrier repair and attenuation of oxidative stress.