剑叶玉簪总黄酮提取及活性研究OA

Using dried and pulverized Hosta ensata flowers as the raw material,the extraction process for total flavonoids was optimized through single-factor experiments combined with orthogonal tests.Results demonstrated that the optimal extraction conditions were a solid-liquid ratio of1∶40,60℃,20 min,and 90%ethanol,achieving a yield of(6.114±0.056)mg/g.Further antioxidant assays revealed that the total fla-vonoids from Hosta ensata exhibited significantly stronger scavenging activity against ABTS+·compared to VC(p<0.05).At lower concen-trations,their DPPH·and·OH scavenging capacities also surpassed those of VC(p<0.05).However,their ferric ion-reducing power was weaker than VC when the concentration exceeded0.1 mg·mL-1(p<0.05)but showed no significant difference at concentrations≤0.05 mg·mL-1(p>0.05).The CCK-8 assay demonstrated that the total flavonoids significantly inhibited the proliferation of gastric cancer BGC823 cells,with an IC50 value of 23.84 μg·mL-1.In conclusion,the total flavonoids from Hosta ensata exhibit remarkable antioxidant and antitumor activities,highlighting their potential for further development in pharmacological or nutraceutical applications.