诱导换羽对老龄种公鸡生殖功能及下丘脑转录组变化的影响OA
Effects of Induced Molting on Reproductive Function and Hypothalamic Transcriptome Changes in Aging Cocks
旨在评估禁食诱导换羽(fasting-induced molting,FM)处理后老龄种公鸡生殖相关表型及下丘脑转录组特征的变化,并分析二者之间的关联.本研究选用60周龄的"豫粉1号"H系公鸡30只,按试验要求随机分为2组,对照组10只,处理组20只.记录处理组禁食前1天(F0)、禁食第15天(F15)和恢复饲喂第39天(R39)时的公鸡体重、血清中高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-Cho)和低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-Cho)的含量,并采集下丘脑组织进行转录组测序(RNA-seq).在R39时,对各组公鸡的精液量、精子密度、精子活力和血清激素水平进行评估.结果表明,FM显著提高了公鸡的精子活力(P<0.05)和密度(P<0.05),尽管促卵泡激素(FSH)、促黄体生成素(LH)和睾酮(T)的水平呈现上升趋势,但差异未达统计显著性(P>0.05).恢复饲喂后体重恢复正常,且LDL-Cho水平显著者上升(P<0.05).RNA测序共识别出14 752个基因,其中7 030个基因表达差异显著,主要涉及神经活性配体-受体相互作用、ECM-受体相互作用、细胞粘附分子和黏着信号通路.禁食期间,神经肽如AGRP、NPY、POMC和GAL上调,而在重新进食后,神经发育相关基因如GPM6B 和 BASP1 上调.免疫相关基因如 CXCL12 和 GRP 在禁食期间表达下调.蛋白相互作用分析揭示了AGT、NTS、TRHR和CCK等基因作为潜在的枢纽基因.本研究结果表明,下丘脑在FM过程中通过神经肽与代谢信号的转录变化,可能间接参与生殖系统功能的恢复.研究为深入理解诱导换羽对老年种公鸡的生殖恢复机制提供了初步的分子基础.
This study aimed to evaluate changes in reproductive-related phenotypes and hypothalamic transcriptomic features in aged breeder roosters following fasting-induced molting(FM)and to analyze the association between them.A total of 30"Yufen No.1"H-line roosters,aged 60 weeks,were randomly assigned to two groups according to the experimental design:a control group consisting of 10 birds and a treatment group consisting of 20 birds.The body weight,serum levels of high-density lipoprotein cholesterol(HDL-Cho)and low-density lipoprotein cholesterol(LDL-Cho)were recorded for the treatment group on the day before fasting(F0),on the 15th day of fasting(F15),and on the 39th day of refeeding(R39).Additionally,hypothalamic tissue was collected for transcriptome sequencing(RNA-seq).On R39,semen volume,sperm density,sperm motility,and serum hormone levels were evaluated for all roosters in each group.The results demonstrated that FM significantly enhanced sperm motility(P<0.05)and density(P<0.05)in the roosters.Although there was an increasing trend in the levels of follicle-stimulating hormone(FSH),luteinizing hormone(LH),and testosterone(T),the differences were not statistically significant(P>0.05).Following refeeding,body weight returned to normal,and LDL-Cho levels showed a significant increase(P<0.05).RNA sequencing identified a total of 14 752 genes,of which 7 030 exhibited significant differential expression.These genes were primarily involved in pathways such as neuroactive ligand-receptor interaction,ECM-receptor interaction,cell adhesion molecules,and adherens signaling.During fasting,neuropeptides such as AGRP,NPY,POMC,and GAL were upregulated,whereas,upon refeeding,neurodevelopment-related genes like GPM6B and BASP1 were upregulated.Immune-related genes such as CXCL12 and GRP were downregulated during fasting.Protein interaction analysis revealed potential hub genes,including AGT,NTS,TRHR,and CCK.The findings of this study suggest that the hypothalamus may play an indirect role in the restoration of reproductive function during the FM process,potentially mediated by transcriptional alterations in neuropeptides and metabolic signaling pathways.This research provides preliminary molecular insights into the reproductive recovery mechanisms induced by molt in aging cocks.
郭海山;刘双星;刘平泉;金炜炜;李烛南;李文婷;康相涛;孙桂荣
河南农业大学动物科技学院,郑州 450046||河南农业大学 神农种业实验室,郑州 450046河南农业大学动物科技学院,郑州 450046||河南农业大学 神农种业实验室,郑州 450046河南农业大学动物科技学院,郑州 450046||河南农业大学 神农种业实验室,郑州 450046河南农业大学动物科技学院,郑州 450046河南农业大学动物科技学院,郑州 450046河南农业大学动物科技学院,郑州 450046||河南农业大学 神农种业实验室,郑州 450046河南农业大学动物科技学院,郑州 450046||河南农业大学 神农种业实验室,郑州 450046河南农业大学动物科技学院,郑州 450046||河南农业大学 神农种业实验室,郑州 450046
农业科技
老龄公鸡下丘脑转录组诱导换羽生殖恢复
aging cockhypothalamustranscriptomeinduced moltingreproduction recover
《畜牧兽医学报》 2026 (6)
3273-3287,15
神农种业实验室一流课题项目(SN01-2022-05)
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