白藜芦醇以JUN为靶点促进成纤维细胞凋亡抑制瘢痕增生的作用研究OA

Objective It is to investigate the mechanism of resveratrol targeting JUN in promoting fibroblast apoptosis and inhibiting scar hyperplasia through transcriptome sequencing and cell experiments.Methods ①Transcriptome sequen-cing and real-time fluorescence quantitative PCR were performed to confirm the differences in the expression levels of fibro-sis gene α-SMA,collagen type Ⅰ,pro-apoptotic gene Bcl-2 and JUN in human hypertrophic scar fibroblasts(HSF)and human dermal fibroblasts(DF).Quantitative fluorescent PCR was used to verify the differences in the mRNA expression levels of JUN in HSF and DF.②HSF cells were collected and divided into the following groups:a control group,a 2 μmol/L resveratrol group,a 10 μmol/L resveratrol group,a 50 μmol/L resveratrol group,and a curcumin A+50 μmol/L resvera-trol group.The mRNA expressions of JUN,Col-Ⅰ,and α-SMA in the cells of the first four groups,and the mRNA expres-sions of p53,Caspase-3,Caspase-9,protein kinase B(Akt),and mammalian target of rapamycin(mTOR)in the cells of all the five groups were detected by RT-qPCR,the protein expressions of Col-Ⅰ and α-SMA in the cells of the first four groups were detected by Western Blot method.Results ①The relative mRNA expressions of JUN,Col-Ⅰ,α-SMA,and Bcl-2 in HSF were significantly higher than those in DF(all P<0.05).②The relative mRNA expressions of JUN,Col-Ⅰ,α-SMA,Akt,and mTOR,as well as the relative protein expressions of Col-Ⅰ and α-SMA in HSF of the 10 μmol/L resveratrol group and 50 μmol/L resveratrol group were significantly lower than those in HSF of the control group(all P<0.05),while the relative mRNA expressions of p53,Caspase-3,and Caspase-9 in HSF of the 10 μmol/L resveratrol group and 50 μmol/L resveratrol group were significantly higher than those in HSF of the control group(all P<0.05),there were no significant differences in the relative mRNA expressions of p53,Caspase-3,Caspase-9,Akt and mTOR between the curcumin A+50 μmol/L resveratrol group and blank group(all P>0.05).Conclusion Resveratrol targeting JUN can promote the apoptosis of fibroblasts and inhibit the occurrence and development of hypertrophic scar.