迷迭香酸/小檗碱无载体自组装纳米颗粒通过调节凋亡相关蛋白抑制卵巢癌细胞增殖与迁移OA

Objective:To explore the effects of self-assembled binary carrier-free nano-medicines(Rosmarinic acid/berberine carrier-free self-assembled nanoparticles,RBNPs)on the proliferation and migration of ovarian cancer cells SKOV3 and CAOV3 in vitro,as well as the underlying molecular mechanisms through regulating apoptosis-related proteins.Methods:We used two ovarian cancer cell lines,SKOV3 and CAOV3,to evaluate the effects of RBNPs on cell viability and long-term proliferation ability through CCK-8 assay,cloning experiments and scratch assays.The effects of RBNPs on cell migration ability were assessed through scratch as-says and Transwell experiments.The expressions of apoptosis-related proteins and proteins relat-ed to the PI3K/AKT/mTOR signaling pathway were analyzed by Western blot.Results:The CCK-8 assay demonstrated that RBNPs could reduce the activity of ovarian cancer cells.The cloning formation experiment indicated that RBNPs could significantly inhibit the proliferation ability of ovarian cancer cells.The scratch test and Transwell test jointly verified that RBNPs could significantly inhibit the migration ability of ovarian cancer cells,ruling out the interference of cell proliferation on the experimental results.The flow cytometry results showed that RBNPs could significantly increase the apoptosis rate of ovarian cancer cells.Western blot analysis re-sults indicated that RBNPs could up-regulate the expression of pro-apoptotic proteins Cleaved Caspase-3 and Bax,down-regulate the expression of anti-apoptotic protein Bcl-xl,and simulta-neously inhibit the activation of the PI3K/AKT/mTOR signaling pathway,confirming that RB-NPs can effectively induce the apoptosis of ovarian cancer cells SKOV3 and CAOV3.Conclu-sion:RBNPs can inhibit the proliferation and migration of ovarian cancer cells by suppressing the PI3K/AKT/mTOR signaling pathway and regulating the expression of apoptosis-related pro-teins.