基于高通量测序探讨子宫内膜异位症患者在位内膜特征基因OA

Objective:To elucidate the gene expression patterns and identify character-istic genes in the eutopic endometrium of endometriosis(EMT)patients by comparing differenti-ally expressed genes(DEGs)between EMT and normal populations,thereby providing new in-sights into the pathogenesis of EMT and important clues for improving reproductive outcomes.Methods:A total of 40 proliferative-phase endometrial samples were collected,including 19 from patients with endometriosis(EMT)and 21 from normal controls.Transcriptomic analysis was performed to identify differentially expressed genes(DEGs)and related pathways.Charac-teristic genes were screened using a protein-protein interaction(PPI)network,and their diag-nostic efficacy was evaluated by receiver operating characteristic(ROC)curve analysis.Finally,we used RT-qPCR to validate the characteristic genes.Result:A total of 127 DEGs were identi-fied,including 55 up-regulated and 72 down-regulated genes.Enrichment analysis revealed that these DEGs were significantly associated with inflammation and immune response pathways.Correlation analysis indicated complex interactions among the DEGs.Through protein-protein interaction network and ROC curve analyses,FCGR3B,GZMB and GNLY were determined as characteristic genes of eutopic endometrium in EMT.Conclusions:This study identifies differ-entially expressed genes between EMT and normal eutopic endometrium by transcriptomic anal-ysis and validates three characteristic genes,offering new clues for understanding the pathophys-iological mechanisms of EMT and aiding in the exploration of potential therapeutic strategies.