豫南地区不同品种花生开花习性分析及AhPEBP基因家族生物信息学分析OA

In order to explore the flowering and podding habits and environmental adaptability of different peanut varieties in Xinyang area of Southern Henan,and to clarify the regulatory role of the flowering－related AhPEBP gene family,this study observed and ana-lyzed the growth and development characteristics,flowering dynamics(initial flowering period,flowering length,flowering amount and peak period)and podding habits of four peanut varieties'Xinhua 425','Yuhua 138','Yuhua 99'and'Yuhua 93'in Southern Henan.Concurrently,the AhPEBP gene family was systematically identified by bioinformatics methods.The results showed that there were significant differences in flowering characteristics among varieties.Four varieties entered the initial flowering stage 27 to 29 days after sowing.with early flowering peak,short flowering period and concentrated flowering,and high peg formation rate.The above research laid a foundation for further understanding the mechanism of peanut flowering regulation was short,the flowering was concentrated,and the needle formation rate was high.Among them,'Yuhua 138'and'Xinhua 425'had the earliest flowering period,'Yuhua 138'had the longest flowering period(31 days),the total number of flowers was the largest,and the rate of needle formation was low(23.38%).'Xinhua 425'had the shortest flowering period(27 days),and the lowest total flower number,but the highest peg－setting rate(29.20%).The AhPEBP gene family identified 30 members,all of which contained conserved domains and were clustered into three subfamilies:FT－like(flowering－promoting),TFL1－like(flowering－inhibiting)and MFT－like.Expression analysis showed that Ah-PEBP24 had the highest expression levels in stamens,pegs and shoot tips,followed by flowers,pistils,pods and shell.In summary,peanut flowering habits are regulated by genetic factors and environmental factors,and AhPEBP24 and AhPEBP29 may be involved in regulating flowering habits.