IQCN在人睾丸和精子中的表达及其与精子活力的相关性分析OA

Objectives:To investigate the expression of the IQCN(IQ motif containing N)gene in human testicular tissue and spermatozoa,and its correlation with human sperm motility. Methods:The mammalian reproductive genetics database was searched to examine the expression of IQCN in testicular tissue and germ cells.Western blotting was used to detect the expression levels of IQCN protein in human testes and sperm,and the protein expression differences of IQCN among different sperm motility groups.Immunohistochemistry and immunofluorescence were employed to study the localization of IQCN expression in human testis and sperm.Bioinformatic analysis was conducted to explore the potential biological functions and related mechanisms of IQCN. Results:IQCN was expressed in both human testicular tissue and sperm,specifically localized in the sperm acrosome.Compared with the normal motility group,the expression level of IQCN was significantly reduced in the asthenozoospermia group.Bioinformatic analysis revealed that IQCN could interact with calmodulin genes such as CALM2,CALM3,CALML3,and PICALM,and may play a role through involvement in biological processes including Ca2+signaling pathways,PKC signaling pathways,and sperm flagellum formation and assembly. Conclusions:IQCN is expressed in human testis and sperm,and its expression differs significantly among groups with varying sperm motility.Abnormal expression of IQCN may lead to decreased sperm motility and male infertility,warranting further in-depth investigation.