清半夏对OVA小鼠的抗炎祛痰作用及机制研究OA

Objective To investigate the anti-inflammatory and expectorant effects and mechanism of the water extract of Pinelliae Rhi-zoma Praeparatum Cum Alumine(PRAWE),a processed product of Rhizoma Pinelliae,in ovalbumin(OVA)-induced asthmatic mice.Methods HE staining,Masson staining,and AB-PAS staining were used to observe the pathological changes in mouse lung tissues.Untargeted metabolomics technology was applied to analyze the metabolic regulation of PRAWE in OVA-induced mice.ELISA kits were used to detect the levels of immunoglobulin E(IgE)in serum,and tumor necrosis factor-α(TNF-α),interleukin(IL)-6,and IL-1β in bronchoalveolar lavage fluid.Western blot(WB)was performed to detect the expression of proteins related to the nuclear factor kappa B(NF-κB)/NOD-like receptor protein 3(NLRP3)signaling pathway in mouse lung tissues.Results Compared with the OVA group,PRAWE treatment at various doses alleviated inflammatory infiltration,reduced collagen fiber deposition,and decreased mucus secre-tion in the lung tissues of mice.PRAWE regulated metabolic disorders in the lung tissues of OVA-induced mice by affecting pathways such as nucleotide metabolism and pyrimidine metabolism.ELISA results showed that compared with the OVA group,PRAWE down-regulated the levels of IgE in serum and TNF-α,IL-6,and IL-1β in bronchoalveolar lavage fluid(P<0.01 or P<0.05).WB results showed that PRAWE significantly inhibited the expression of phosphorylated(p)-p65,p-inhibitor of kappa B alpha(IκBα),NLRP3,apoptosis-associated speck-like protein containing a CARD(ASC),and caspase-1(P<0.05 or P<0.01).Conclusion PRAWE can effectively alleviate inflammation,reduce mucus secretion,and regulate metabolic disorders in OVA-induced mice.It exerts anti-inflammatory and expectorant effects in OVA-induced asthmatic mice by influencing the NF-κB/NLRP3 signaling pathway.