减味寿胎丸对流产模型大鼠母胎界面血管生成功能的影响OA

Objective To explore the effect of Jianwei Shoutai Pill(减味寿胎丸,JWSTP/JW)on the angiogenic function at the maternal-fetal interface in the rats model of abortion.Methods The rats model of abortion were established by induction of mifepristone.The rats were then treated with dydrogesterone or JWSTP.The rats were divided into the control group(Control),the abortion model group(Model),the positive control group treated with dydrogesterone(Positive),and the group treated with JWSTP.Except for the Control group,the other groups were given 1.2 mg/mL mifepristone solution by gavage on the 13th day of pregnancy to establish the abor-tion model.From the 0th to the 14th day of pregnancy,the Control and Model groups were given 0.5%CMC-Na solution by gavage,the Positive group was given 0.54 mg/mL dydrogesterone solution by gavage,and the JW group was given 180 mg/mL JWSTP solution by gavage.The uterine and embryo morphology of each group was observed under a stereomicroscope,and the embryo loss rate was calcu-lated.The stroke volume of umbilical blood flow in pregnant rats was measured by small animal ultrasound.The expression of angio-genic factors and Notch-1/Dll4,PI3K/AKT pathway proteins in the decidua tissue of pregnant rats in each group was detected by immu-nohistochemistry(IHC)and Western blot(WB).Results The uterus of the Control group was light red in color,with a thick and bead-like shape.No pronounced blood stasis points were observed in the uterine cavity and embryo implantation sites,and the embryos were plump and rosy with clear blood vessels and branches,and rich blood supply.Compared with the Control group,the uterus of the Model group was dark in color,with some segments of the uterus showing no embryos,and a bamboo-like shape.When dissected,scattered blood stasis points were observed in the uterine cavity and embryo implantation sites,and the embryos themselves had blood stasis points,were shriveled,and pale in color,with significantly reduced blood vessels and branches.Compared with the Model group,the uterus of the Positive and JWSTP groups was significantly more rosy in color and regular in shape.When dissected,only a few scattered blood stasis points were observed in the uterine cavity and embryo implantation sites,and the embryos were plump,with a reddish color,and obvious blood vessels and branches.Compared with the Control group,the embryo loss rate in the Model group was signifi-cantly increased,the stroke volume of umbilical blood flow decreased significantly,and the expression of VEGFA,VEGFR1,and VEGFR2 proteins was significantly downregulated in IHC and WB(P<0.05),in WB,the expressions of Notch-1,PI3K and AKT were all decreased(P<0.05),while there was no significant difference in the expression of Dll4;Compared with the Model group,the embryo loss rate in the Positive and JWSTP groups was significantly decreased,the stroke volume of umbilical blood flow has signifi-cantly increased,and the expression of VEGFA,VEGFR1,and VEGFR2 proteins was significantly increased in IHC and WB(P<0.05);Compared with the Model group,there were no significant differences in the expression of Notch-1 and Dll4 in the Positive group,while the expressions of PI3K and AKT significantly increased;compared with the Model group,the expressions of Notch-1 and PI3K,AKT in the JWSTP group significantly increased,while the expression of Dll4 showed no significant difference.Conclusion JWSTP can significantly reduce the embryo loss rate in the mifepristone-induced rats abortion model.It is speculated that the reduction of teratogenic effects in JWSTP may occur through the Notch and PI3K/AKT pathways,thereby influencing the vascular growth function and preventing the occurrence of spontaneous abortion.