基于TLC-HPLC的多基原臭茉莉质量控制方法建立与评价OA

Objective Clerodendrum phillppinum Schauer var.simplex(Clerodendrum)is a genuine medicinal material in Lingnan,and its quality standard is imperfect.In this paper,the quality control method of Clerodendrum is systematically studied to provide a basis for the formulation of its quality standard.Methods A total of 30 batches of Clerodendrum medicinal materials from Lingnan area were collected,contents of water,total ash,and water-soluble extract of Clerodendrum were determined according to the 2025 edition of Chinese Pharmacopoeia(中国药典).To establish a thin layer chromatography(TLC)identification method for Clerodendrum,based on high-performance liquid chromatography(HPLC),the fingerprint and the content determination method of verbascoside were estab-lished.Results The average contents of water,total ash content and water-soluble extract content of 30 batches of Clerodendrum were 4.60％,3.25％and 15.02％,respectively.The established TLC method was good,and the results showed that multiple batches of Clerodendrum showed the same color spots at the same Rf.The established HPLC method was stable in the linear range of 0.004-0.101 mg·mL-1(r=1.000).The content of verbascoside in 30 batches of Clerodendrum was 0.021-0.738％.The fingerprints of multiple batches of Clerodendrum were similar.5 common peaks were identified in the fingerprints,and the 2 common peaks were identified as verbascoside and isoverbascoside.Conclusion The quality control methods such as content determination and fingerprint of Cleroden-drum were established,which provided the key experimental basis for the improvement of the quality standard of Clerodendrum.