参芪地黄汤化裁方含药血清对高糖诱导的小鼠永生化足细胞焦亡的保护作用影响OA

Objective To investigate the protective effect of serum containing Shenqi Dihuang Decoction modified prescription(参芪地黄汤,SQDH)against pyroptosis in immortalized mouse podocytes induced by high glucose.Methods Mouse podocytes(MPC5)were cultured in vitro.Optimal intervention concentrations of high glucose and SQDH-containing serum,as well as exposure duration,were determined.Cells were divided into four groups:normal control,high glucose model,SQDH-containing serum,and NLRP3 inhibitor control(MCC950 group).Morphological changes indicative of pyroptosis were observed under light microscopy.Levels of IL-1β and IL-18 in cell supernatants were measured by enzyme-linked immunosorbent assay(ELISA).Protein expression of Nephrin,Podocin,PODXL,WT-1,Desmin,NLRP3,Cleaved Caspase-1,and GSDMD-N was detected via Western blot(WB).Immunofluorescence was used to assess expression levels of Nephrin,NLRP3,GSDMD-N,and Cleaved Caspase-1.Results Compared with the normal control group,the high glucose model group exhibited disrupted cell membrane integrity and reduced cell density.ELISA results showed upregulated expression of IL-1β and IL-18(P<0.05).Western blot analysis revealed decreased expression of Nephrin,Podocin,PODXL,and WT-1(P<0.05),and increased expression of Desmin,NLRP3,Cleaved Caspase-1,and GSDMD-N(P<0.05).Immu-nofluorescence indicated elevated relative fluorescence intensity of NLRP3,Cleaved Caspase-1,and GSDMD-N(P<0.05),alongside reduced Nephrin expression(P<0.05).Both the SQDH-containing serum group and MCC950 group showed significant amelioration of these abnormalities compared to the model group(P<0.05).Conclusion Serum containing SQDH modified prescription alleviates high glucose-induced podocyte injury,potentially by mitigating pyroptosis.