基于氧化应激及PI3K/AKT通路的补气活血益精方改善肾虚血瘀型少弱精子症大鼠睾丸组织的研究OA

Objective To investigate the ameliorative effect of Buqi Huoxue Yijing Formula(补气活血益精方,BHYF)on oxidative stress damage in the testes of rats with kidney deficiency and blood stasis-type oligoasthenospermia(OAS)and its regulatory mechanism on the phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway.Methods Sixty male SD rats were randomly divided into a blank group(n=10)and a modeling group(n=50).The model was established by intragastric administration of Tripter-ygium glycosides(40 mg·kg-1·d-1).After successful modeling,the rats were further divided into the model group,L-carnitine group(1.8 mL·kg-1·d-1),and low/medium/high-dose BHYF groups(7.87,15.74,and 31.5 g·kg-1·d-1,respectively).Treatments contin-ued for 28 days.At the end of the 8-week experimental period,bilateral testicular tissues were harvested.The following indicators were assessed:the general condition of rats;sperm quality using an automated sperm analyzer;histopathological changes in testicular tissue by hematoxylin-eosin(HE)staining;levels of reactive oxygen species(ROS)and malondialdehyde(MDA)in testicular tissue by enzyme-linked immunosorbent assay(ELISA);and protein expression of PI3K,p-PI3K,AKT,and p-AKT by Western blot(WB).Results Compared with the blank group,the model group exhibited significantly reduced sperm concentration and motility,structural disruption of seminiferous tubules,markedly elevated levels of ROS and MDA(P<0.01),and significantly decreased expression of PI3K,p-PI3K,AKT,and p-AKT proteins(P<0.01).All treatment groups(especially the high-dose group)effectively restored semi-niferous tubule structure and significantly reduced ROS and MDA levels(P<0.01,P<0.05).Except for the low-dose group,all other treatment groups significantly upregulated the expression of PI3K/AKT pathway-related proteins(P<0.01).Conclusion BHYF may improve testicular spermatogenic function by regulating oxidative stress and activating the PI3K/AKT signaling pathway.