菌藻-植物联合修复提升矿山渣土功能并重组微生物群落OA

[Objective]The utilization of microorganisms and plants to improve mine waste soil has certain potential.This study aimed to investigate the effects of microbial inoculants and cyanobacterial solutions under different plant colonization conditions on soil fertility,enzyme activities,and microbial communities,and to provide a scientific basis for the biological amelioration of nutrient-poor mining substrates.[Methods]Using mine waste soil as the object,nine treatment combinations were established in a pot experiment:Nostoc+Gramineous plants(NG),compound microbial agent(Arbuscular mycorrhizal fungi+Tricho-derma harzianum)+Gramineous plants(MG),microbial agent-cyanobacteria(compound microbial agent+Nostoc)+Gramin-eous plants(MNG),Nostoc+Leguminous plants(NL),compound microbial agent+Leguminous plants(ML),compound microbial agent-cyanobacteria+Leguminous plants(MNL),Nostoc+Gramineae-Leguminosae mixed sowing(NGL),com-pound microbial agent+Gramineae-Leguminosae mixed sowing(MGL),and compound microbial agent-cyanobacteria+Gra-mineae-Leguminosae mixed sowing(MNGL).A no-planting treatment served as the control(CK).Soil nutrient contents,soil enzyme activities,and the community structure and diversity of fungi and bacteria were systematically analyzed under differ-ent treatments.[Results]Compared with the CK,the MNG and MNGL treatments significantly increased soil organic matter content by 9.4%～13.1%.The MNG treatment significantly enhanced the contents of available phosphorus and available po-tassium.Sucrase activity under the NL,ML,and MNL treatments was significantly higher than that in other treatments,in-creasing by 2.06～2.87 times compared with the CK.Alkaline phosphatase activity showed the largest increase under the MNL treatment,significantly increasing by 96.6%compared with the CK.A total of 3985 fungal ASVs were detected,of which only 1.65%were shared core taxa,while 9729 bacterial ASVs were detected,with shared core taxa accounting for 8.82%.Under the MNL and MNGL treatments,the primary dominant fungal phylum in the soil was Ascomycota,and the primary dominant bacterial phyla included Proteobacteria and Bacteroidota.The cyanobacteria-microbial agent treatments resulted in lower fungal community richness and diversity indices than the CK,and driven their community structures to converge under different plant configuration conditions,while bacterial community diversity and structural changes were minor.These shifts were closely asso-ciated with soil physicochemical properties and enzyme activities,with a more pronounced influence observed on fungal commu-nities.In the comprehensive soil quality evaluation,the MGL,MNGL,and NGL treatments ranked as the top three.[Conclu-sion]The synergistic colonization of compound microbial agents,cyanobacteria,,and different plants had significant effects on the nutrients,enzyme activities,and microbial communities of mine waste soil.Among them,the"compound microbial agent-cyanobacteria+Gramineae-Leguminosae mixed sowing"model showed the optimal effect for improving mine waste soil.