低速与高速离心机处理后脂血标本的生化检验结果分析OA

Objective:To explore the difference of biochemical test results of lipid blood samples treated with differ-ent centrifugation speeds,and to provide basis for optimizing the treatment scheme of lipid blood samples.Meth-ods:Thirty samples of hyperlipemia received by Yangjiang Center for Disease Control and Prevention in Guangdong Province from March 2021 to December 2024 were selected.Each sample was first treated by L4-5K low-speed centrifuge(3000 rpm,10 min),and the serum was separated and divided into two tubes,one of which was to be tested for later use,and the other was treated by MIKRO-220R high-speed freezing centrifuge(13000 rpm,10 min).Ten indexes,such as ALT、AST、GLU、CR、UA、BUN、TG、CHOL、HDL-C、LDL-C,were detected by automatic biochemical analyzer,and the detection results at two centrifugal speeds were statistically analyzed by Wilcoxon signed rank test.Results:Among the 10 indexes,there were significant differences in seven indexes:the results of high-speed centrifugation of GLU、UA、TG and CHOL were lower than those of low-speed centrifuga-tion(P<0.001);LDL-C and HDL-C showed a significant decreasing trend(P<0.001).The decreasing trend of BUN is not as obvious as other indicators(P=0.032).After high-speed centrifugation,the results of CR in-creased significantly(P=0.001).The differences of the above indexes were statistically significant(P<0.05).There was no significant difference between AST and ALT(P>0.05).Conclusion:High-speed centrifugation can effectively eliminate the light scattering interference of lipid blood on many biochemical detection items,and correct the false low CR,so it is suggested to use it as a treatment method for lipid blood samples.