骨乐冲剂抑制成骨细胞程序性坏死机制研究OACHSSCD

Objective:To investigate the mechanisms by which Gule Infusion Granules inhibit necroptosis in osteoblasts.Methods:Osteoblasts were isolated from the calvaria of neonatal rats,and a cellular model of osteoblast necroptosis was established by treat-ment with resibufogenin(RBG).The cells were divided into a control group without intervention,a model group treated with 20 μmol/L RBG,and a Gule Infusion Granules group treated with 20 μmol/L RBG in combination with high-dose Gule Infusion Granules-containing serum.Cell viability,alkaline phosphatase(ALP)activity,intracellular ultrastructural changes,mitochondrial permeability transition pore(MPTP)opening,reactive oxygen species(ROS)levels,and the expression of proteins associated with the RIPK1/RIPK3/MLKL signaling pathway were evaluated and compared among the groups.Results:Compared with the control group,osteoblasts in the RBG model group exhibited significantly reduced cell viability and ALP activity.In contrast,ROS levels,MPTP opening,and the expression levels of the key necroptosis-related proteins RIPK1,RIPK3,and MLKL were significantly in-creased.Treatment with Gule Infusion Granules significantly reversed these changes.Transmission electron microscopy further dem-onstrated that cells in the model group displayed typical ultrastructural features of necroptosis,whereas these morphological altera-tions were markedly improved in the Gule Infusion Granules group.Conclusion:Gule Infusion Granules inhibits osteoblast necrop-tosis and promotes osteoblast differentiation by activating the RIPK1/RIPK3/MLKL signaling pathway.