基于指纹图谱与化学模式识别的多基原威灵仙质量评价研究OACHSSCD

Objective:To establish fingerprint profiles of Clematidis Radix et Rhizoma derived from different botanical origins,in-vestigate their quality differences and classification characteristics,and provide an experimental basis for the quality evaluation of Clematidis Radix et Rhizoma.Methods:High-performance liquid chromatography(HPLC)was employed to establish fingerprint profiles of Clematidis Radix et Rhizoma from different botanical origins.Similarity evaluation was performed,and cluster analysis(CA),principal component analysis(PCA),and orthogonal partial least squares discriminant analysis(OPLS-DA)were applied to classify 26 batches of medicinal materials and identify the major components contributing to their classification.Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q/TOF-MS)was used to characterize the chemical constituents of Clematidis Radix et Rhizoma.Results:Fingerprint profiles were established for 26 batches of Clematidis Radix et Rhizoma collected from different production areas and representing different botanical origins.A total of 22 common finger-print peaks were identified.Using peak 8(hederagenin)as the reference peak,the similarity values ranged from 0.219 to 1.000.CA,PCA,and OPLS-DA consistently classified the 26 batches into four groups.Seven characteristic chromatographic peaks,namely peaks 14,19,11,22,12,8,and 13,were identified as the major contributors to the classification.UPLC-Q/TOF-MS analysis identi-fied 32 compounds,including daidzein,magnoflorine,hesperetin,and nobiletin.Conclusion:Clematidis Radix et Rhizoma from dif-ferent botanical origins exhibits significant differences in chemical composition.These characteristics are closely associated with both botanical origin and production area.This study provides a scientific basis for the quality evaluation of Clematidis Radix et Rhizoma.