基于网络药理学和实验验证黄芪赤风汤治疗脑梗死的作用机制OACHSSCD

Objective:To investigate the molecular mechanisms underlying the therapeutic effects of Huangqi Chifeng Decoction on cerebral infarction using network pharmacology and animal experiments.Methods:Network pharmacology was employed to identify the active components of Huangqi Chifeng Decoction and their potential targets associated with cerebral infarction.Molecular doc-king was performed to verify the interactions between the major active compounds and core targets,and the findings were further validated through animal experiments.Results:A total of 56 active compounds and 298 targets were identified.Molecular docking a-nalysis demonstrated that five key compounds could directly interact with seven core targets,and the core compounds exhibited fa-vorable binding affinities with the core proteins.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis indicated that Huangqi Chifeng Decoction may exert therapeutic effects on cerebral infarction primarily through the phosphoinositide 3-kinase/protein kinase B(PI3K/AKT1)signaling pathway,tumor necrosis factor(TNF)signaling pathway,interleukin-17(IL-17)signaling pathway,lipid and atherosclerosis pathway,advanced glycation end product-receptor for advanced glycation end product(AGE-RAGE)signaling pathway,and fluid shear stress and atherosclerosis pathway.Experimental validation demonstrated that Huangqi Chifeng Decoction improved neurological deficits in rats,reduced the levels of IL-6,TNF,and IL-1β in peripheral serum,and improved the expression of AKT1 and TNF in brain tissue.Conclusion:The therapeutic effects of Huangqi Chifeng Decoction on cerebral infarction may be associated with the regulation of core targets,including AKT1,TNF,IL-6,and IL-1β.