五子衍宗丸通过甲酰肽受体2通路抑制神经炎症OACHSSCD

Objective:To investigate the effects of Wuzi Yanzong Pills(WYP)on MPTP-induced neuroinflammation in mice and explore its regulatory role in the formyl peptide receptor 2(FPR2)-resolvin D1(RvD1)/p38 mitogen-activated protein kinase(p38 MAPK)/nuclear factor κB(NF-κB)signaling pathway.Methods:Sixty mice were randomly assigned to four groups:control(Con),MPTP,WYP,and positive control(L-DOPA).Behavioral assessments were performed to evaluate motor coordination.Immunofluo-rescence(IF)was used to detect tyrosine hydroxylase(TH),FPR2,ionized calcium-binding adapter molecule 1(Iba1),and NF-κB in brain tissue.Levels of FPR2,RvD1,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and interleukin-1 β(IL-1β)in brain homogenates were measured using enzyme-linked immunosorbent assay(ELISA).Quantitative real-time PCR(qRT-PCR)assessed mRNA expression of FPR2,Iba1,and p38,while Western blot(WB)analyzed protein expression of TH and FPR2.Results:Com-pared with the Con group,MPTP-treated mice showed impaired motor coordination,increased expression of FPR2,TNF-α,IL-6,IL-1 β,Iba1,p38,and NF-κB in brain tissue(P＜0.05),and decreased expression of TH and RvD1(P＜0.05).Compared with the MPTP group,WYP treatment improved motor coordination,decreased TNF-α,IL-6,IL-1 β,Iba1,p38,and NF-κB expression(P＜0.05),and increased TH expression(P＜0.05).Notably,FPR2 expression was further elevated in the WYP group compared to the MPTP group,accompanied by a significant increase in its ligand,RvD 1.Conclusion:WYP inhibits MPTP-induced neuroinflamma-tion in mice by enhancing the specific interaction between FPR2 and RvD1,thereby modulating the p38 MAPK/NF-κB signaling pathway.