半夏泻心汤对胃癌细胞凋亡的作用机制研究OACHSSCD

Objective:To investigate the effects of Banxia Xiexin Decoction on N6-methyladenosine(m6A)methylation in MKN-45 gastric cancer cells and its mechanisms in promoting apoptosis.Methods:Thirty rats were randomly divided into three groups(n=10 each)according to the random number table method:blank serum group,western medicine group,and Banxia Xiexin Decoction group.Rats were treated for 2 weeks with intragastric administration of Banxia Xiexin Decoction,intragastric administration of nor-mal saline,or tail vein injection of 5-fluorouracil(5-FU),respectively.After the final administration,blood was collected from the abdominal aorta to prepare drug-containing serum.The m6A RNA methylation level in MKN-45 cells was measured by colorimetric assay.RT-PCR was used to detect mRNA expression of methyltransferase-like 3(METTL3),B-cell lymphoma-2(Bcl-2)-associated X protein(Bax),Bcl-2,and Caspase-3.Protein expression of METTL3,Bax,Bcl-2,and Caspase-3 was assessed by Western blot.Results:Compared with the blank serum group,Banxia Xiexin Decoction significantly decreased METTL3 expression and m6A RNA methylation levels in MKN-45 cells,reduced Bcl-2 mRNA and protein expression,and increased Bax and Caspase-3 mRNA and protein expression(P＜0.05).Conclusion:Banxia Xiexin Decoction may exert anti-tumor effects by downregulating METTL3-mediated m6A methylation,thereby upregulating pro-apoptotic proteins Bax and Caspase-3 and downregulating the anti-apoptotic protein Bcl-2.