四君子汤对高压电烧伤大鼠免疫功能及血管内皮生长因子表达的影响OACHSSCD

Objective:To investigate the effects of Sijunzi Decoction on immune function and vascular growth factors in rats with high-voltage electrical burns.Methods:A total of 120 healthy male SD rats were randomly assigned into four groups(n=30 each):sham injury group,model group,low-dose Sijunzi Decoction group,and high-dose Sijunzi Decoction group.Rats in the sham injury group did not receive electric shock,while a high-voltage electrical burn model was established in the other groups.After modeling,rats in the low-and high-dose groups received Sijunzi Decoction extract via gavage at 2.86 g/kg and 5.72 g/kg,respectively,while the sham injury and model groups received an equal volume of saline.Blood samples and immune organs were collected at 48 h,72 h,and 7 d after modeling.Serum immunoglobulins(IgG,IgA,IgM),inflammatory cytokines,vascular endothelial growth factor(VEGF),platelet-activating factor(PAF),and thrombopoietin(TPO)were measured using commercial kits.STAT3 mRNA and VEGF mRNA expression levels in serum were determined by reverse transcription quantitative PCR.Results:Compared with the sham injury group,the model group exhibited significantly decreased thymus and spleen indices and lower serum IgG,IgA,and IgM levels,along with significantly elevated serum pro-inflammatory cytokines,VEGF,PAF,and TPO levels(P＜0.05).Compared with the model group,rats in the low-and high-dose Sijunzi Decoction groups showed significant increases in thymus and spleen indices and serum IgG,IgA,IgM,and VEGF levels,and significant decreases in pro-inflammatory cytokines,PAF,and TPO levels(P＜0.05).Conclusion:Sijunzi Decoction may alleviate high-voltage electrical burn injury by upregulating STAT3 to enhance VEGF expression and promote healing,inhibiting PAF and TPO to reduce vascular injury,and improving immune function.