戊糖片球菌368对杜撒猪粪便中真菌、肉品质和组织脂溶性维生素含量的影响OA

The study aimed to explore the effects of Pediococcus pentosaceus 368 in the fecal fungi,meat quality,and fat-soluble vitamin of Duroc×Saba pig.Twenty Duroc×Saba pigs which body weight were about 77 kg were randomly divided into 2 groups with 10 pigs per group which was used the single-factor experi-mental designp.The pigs in experimental group(group FA)were drinked with the water adding Pediococcus pentosaceus 368(1010 CFU/L).The experiment lasted for 28 d.On the day of the end of the experiment,slaughterings were conducted.The effects of Pediococcus pentosaceus 368 on Duroc×Saba pigs were ana-lyzed through the meat quality,fecal fungi,and the analysis of fat-soluble vitamin content.The results showed as follows:① principal coordinates analy-sis(PCoA)revealed that samples from the two groups were not completely separated.After adding Pediococcus pentosaceus 368,the species similarity in the experimental group tended to be consistent.② At the phylum level,the highest rela-tive abundance in the control group was Mucoro-mycota,while in the experimental group,it was Chytridiomycota.At the genus level,there were 20 genera with a relative abundance greater than 1.0%.The highest relative abundance in both groups was Rhizopus.③ The CAZy(Carbohydrate-Active enZYmes Database)level functional analysis found that at the second level,the dominant CAZy gene functions were annotated into 20 major pathways.④ Meat quality analysis showed that there was no significant(P>0.05)among two groups.Amino acid composition analysis revealed no significant differences between the two groups(P>0.05).⑤ Metabolomics analysis found that the content of vitamin A in liver and all-trans retinol in the kidneys significantly increased(P<0.05),while the content of α-tocopherol and β-tocopherol in the serum significantly decreased(P<0.05).In summary,adding Pedio-coccus pentosaceus 368 to the drinking water of Duroc×Saba pig led to a consistent species composition of fungi in the feces,increased the content of vitamin A in liver and all-trans retinol in the kidneys,and decreased the content of α-tocopherol and β-tocopherol in the serum,demonstrating probiotic functions in enhancing amino acid and fat metabolism-related bacteria,enzymes and complex carbohydrate utilization.