月季IQD基因家族鉴定及表达分析OA

The IQD(IQ67-domain)proteins represent a class of plant-specific calmodulin(CaM)-binding proteins that participate in calcium signaling through their conserved IQ domains,playing critical roles in plant growth and development,stress responses,and secondary metabolism.To systematically analyze the distribution and potential functions of the Rosa chinensis IQD gene family,23 IQD genes were identified in the Rosa chinensis genome.Subsequent investigations were conducted to characterize their physicochemical properties,chromosomal localization,gene structures,promoter cis-acting elements,expression levels,and phylogenetic relationships of IQD proteins.The results demonstrated that Rosa chinensis IQD proteins exhibited high isoelectric points and hydrophilic properties,with uneven distribution across seven chromosomes.The Rosa chinensis IQD protein family could be divided into five subfamilies(Ⅰ-Ⅴ),with 47.82％(11/23)of Rosa chinensis IQD proteins clustering into a single branch with two Arabidopsis thaliana IQD proteins.Rosa chinensis IQD genes contained 3-7 exons,with all Rosa chinensis IQD proteins harboring conserved Motif 1 and Motif2.Among Rosa chinensis IQD genes,the highest number(9-24)of promoter cis-acting elements was associated with"abiotic and biotic stress responses",while those related to"plant growth and development"ranged from 3 to 19.The expression levels of Rosa chinensis IQD genes varied across different tissues.Furthermore,16 Rosa chinensis IQD genes exhibited distinct expression levels under high-temperature stress,demonstrating their responsiveness to thermal challenges.These findings provide a theoretical basis for elucidating the functional roles of Rosa chinensis IQD genes and their regulatory mechanisms in stress adaptation.