桃PP2C基因家族全基因组鉴定及表达分析OA

PP2C(Type 2C protein phosphatase)is a class of ubiquitously present protein phosphatases that play critical roles in various physiological processes in the plants,including stress response and hormone signaling.However,the research on PP2C in peach(Prunus persica)remains limited and requires further ex-ploration.In this study,using'21 Shiji'peach as experimental material,a total of 56 genes harboring an in-tact PP2C conserved domain were identified from the peach genome through bioinformatics methods.The length of their encoded proteins ranged from 169 to 1 080 amino acids,and the molecular weight of these pro-teins ranged from 19.20 kDa to 119.48 kDa.Phylogenetic analysis revealed that the 56 PpPP2C proteins could be classified into seven subfamilies.Tissue-specific expression analysis showed that PpPP2C genes exhibited distinct expression patterns across different peach tissues.PpPP2C40 was predominantly expressed in stems,PpPP2C2,PpPP2C9,PpPP2C14,PpPP2C26,PpPP2C30,PpPP2C48 and PpPP2C51 showed the highest expression levels in buds,and PpPP2C12 and PpPP2C43 were the most highly expressed in leaves.Further-more,one-year-old potted peach seedlings were subjected to 0 ℃ low-temperature treatment,and RT-qPCR a-nalysis revealed that the expression of two PpPP2C genes were up-regulated while that of eight genes were down-regulated,suggesting functional divergence among family members in response to cold.This study pro-vided the first systematic identification of peach PpPP2C gene family,revealed that certain genes specifically expressed in buds and leaves might be involved in organ development,and demonstrated that most members were cold-responsive with suppressed expression under low-temperature stress.These findings could provide a reference for future in-depth functional research of PP2C genes in peach.