花生DOG1基因家族的鉴定与表达分析OA

The DOG1(Delay of germination 1)gene is an important regulatory gene controlling seed dormancy.In order to explore the mechanism of peanut seed dormancy,a total of 35 DOG1 gene family mem-bers were identified from the whole genome of cultivated peanut(Arachis hypogaea L.)in this study,and the physicochemical properties,phylogenetic evolution of their coding proteins and the gene expression patterns were analyzed.The results showed that the number of amino acids encoded by DOG1 gene family members ranged from 126 to 662;the DOG1 proteins had the molecular weight ranging from 15.122 to 75.166 kDa,the theoretical isoelectric point between 5.16 and 8.79,and about 68.6％of them were weakly acidic proteins,while all were hydrophilic proteins.Phylogenetic evolution analysis showed that the 35 DOG1 gene family members were distributed on 18 chromosomes and could be divided into 7 subfamilies.The DOG1 genes showed tissue expression specificity.Four genes(Ah0HHX3D,Ah5PG8C6,AhE6CWZU and AhDXWW21)with kernel-specific expression were selected for fluorescence quantitative PCR(RT-qPCR)analysis and their expression levels at 1,3,5 and 7 d of germination in H7500 and ZP06 peanut seeds were analyzed.The re-sults showed that the expression level of Ah0HHX3D and AhE6CWZU showed the gradually decreasing trend in the embryos of germinated seeds,but were always at a higher level in non-germinated seeds,proving that they might be involved in the process of seed germination.These results could lay a foundation for further research on the function of DOG1 gene family in seed germination process of peanut.