甘蓝型油菜ABI3基因的克隆、表达分析和功能的初步研究OA

[Objective]To clone the coding sequence(CDS)of the Brassica napus ABI3 gene,analyze the structural features of the BnABI3 gene,the properties of its encoded protein,and its expression pat-tern under abiotic stress,thereby providing insights into the regulatory role of the ABI3 gene in respond-ing to abiotic stress.[Method]Using leaf cDNA from Brassica napus"Zhongyou 821"as a template,the BnABI3 gene CDS sequence was cloned.Bioinformatics analysis was performed,and its expression under abiotic stress was analyzed using qRT-PCR.Protein interactions were verified through Y2H and BiFC ex-periments.[Result]The CDS region of BnABI3 gene in B.napus was 2 133 bp in length,encoding 710 amino acids.The homology with AtABI3 protein sequence in A.thaliana was 84.33%.The BnABI3 gene responds to drought,low temperature,and salt stress treatments,exhibiting sharply induced expression under salt stress with a response intensity higher than other stress treatments.The BnABI3 protein local-ized to the nucleus,and yeast two-hybrid and bimolecular fluorescence complementation analyses indi-cated its interaction with the transcription factor BnABI5.[Conclusion]BnABI3 is a nuclear-localized protein that likely mediates BnABI5-regulated biological activities through interaction with BnABI5,par-ticipating in Brassica napus responses to drought,low temperature,and salt stress.These findings pro-vide insights into the regulatory mechanisms of BnABI3 stress resistance and lay the foundation for further functional studies of the BnABI3 gene.