榴莲花粉活力测定方法的比较研究OA

In this study,two durian cultivars,'MonThong'(Jinzhen)and'Musang King'(Maoshanwang),were used as ex-perimental materials.Pollens at the full bloom stage were collected,and the pollen viability of durian stored for 2,4,8,12,18,and 24 hours was determined by the Alexander staining method and MTT staining method.In addition,the pollen germination rates at different culture times were observed via ex-vitro germination method(solid and liquid ex vitro germination methods).The results showed that the pollen viability of both cultivars showed a decreasing trend with the extension of storage time,with no significant difference within 2-4 hours;the pollen viability of'MonThong'(Jinzhen)was higher than that of'Musang King'(Maoshanwang)from 8 to 12 hours,and the pollen viability was basically lost after 24 hours,indicating that pollen storage under normal temperature and dry conditions is only suitable for short-term storage within 8 hours.The Alexander staining method is more suitable for the rapid detection of durian pollen viability and its level,while the ex-vitro germination method,although time-consuming,has more accurate and reliable results;the combination of the two methods can improve the detection accuracy.This study provides a scientific basis for optimizing the timing of artificial durian pollination,formulating pollen preservation strategies,and promoting hybrid breeding.