珍稀植物蒜头果外植体消毒及诱导启动培养研究OA

Taking the terminal buds and axillary buds of M.oleifera as experimental materials,aseptic disinfection technol-ogy and induction initiation culture technology for M.oleifera explants were studied.The results showed that the optimal dis-infection method for M.oleifera explants was as follows:disinfected with 75%alcohol for one min and rinsed twice with ster-ile water.the samples were centrifuged at a speed of 3 000～4 000 r·min-1 for five min,disinfected in NaClO for 5 min,and rinsed 5 times with sterile water.Then the samples were placed in a glass bottle containing 0.1%HgCl2,disinfected for 10 min,and finally rinsed five times with sterile water.The explants treated by this disinfection method had a low contamination rate,which was conducive to explant transfer and initiation culture.The most suitable medium for M.oleifera was induction initia-tion culture with modified MS+0.2 mg·L-1 6-BA+0.2 mg·L-1 NAA.The plants induced by this medium grew the fastest,had the most branches.