不同方式处理八角茴香种子对其萌发特性的影响OA

To understand the germination characteristics of Illicium verum seeds,the effects of different storage methods,low-temperature treatment durations,and exogenous hormone concentrations on germination were explored of I.verum seeds and provide a scientific basis for standardized seedling propagation technology for the treatment of this species.Taking I.verum seeds as materials,their water absorption characteristics were determined.Three storage methods(ambi-ent-temperature storage,moist-sand storage,and low-temperature storage),three exogenous hormones(IAA,GA3,and NAA)with four concentrations(500,1 000,1 500,and 2 000 mg·L-1),followed by moist-sand storage,and different low-temperature treatment durations followed by moist-sand storage were set up to study the effects of different storage methods,different hormone concentrations,and different low-temperature treatment durations on seed germination poten-tial,germination rate,and germination index.Results show that,after soaking I.verum seeds for 48 hours,the water ab-sorption rate was only 30.05%,and the seed coat permeability was poor,showing significant seed dormancy characteristics.The moist-sand storage method performed excellently in comprehensive performance,with a germination rate of 65%,while the ambient-temperature storage was only 7.33%,and the low-temperature storage was 28.33%.All hormone-treated seed groups had a significantly higher germination rate,germination potential,and germination index than the control group(CK),among which gibberellin(GA3)had the most significant promoting effect.The 500 mg·L-1 gibberellin treat-ment group was significantly superior to the other treatment groups and the control group in germination rate(77.17%),germination potential(41.84%),and germination index(4.30).Cold storage treatment for 10-15 days could significantly improve germination efficiency.I.verum seeds have weak water absorption capacity and poor seed coat permeability,showing significant seed dormancy characteristics.After harvest,seeds are not suitable for long-term ambient-temperature or low-temperature storage,while under moist-sand storage conditions,seed viability is well maintained.The germination effect of I.verum seeds is the best under the conditions of 15-day low-temperature treatment,500 mg·L-1 gibberellin seed soaking,and moist sand storage.