RcGSTF11作为潜在光合调节物的双效调节机制研究OA

To investigate the mechanism through which GSTF acts as a potential photosynthetic regulator in photo-synthesis,this study overexpressed the castor bean RcGSTF11 gene in Arabidopsis thaliana.It was found that the plant height and silique yield of RcGSTF11-OE Arabidopsis plants were significantly higher than those of the wild type,and the net photosynthetic rate(Pn)increased by approximately 28.31%.The interaction between Rc GSTF11 and the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco),Rc RBCL,was verified by surface plasmon reso-nance(SPR)and bimolecular fluorescence complementation(BiFC)assays.The Rubisco activity in RcGSTF11-OE Ara-bidopsis plants significantly increased.Combined analysis of transcriptomics and metabolomics enriched the differen-tially expressed genes in photosynthetic antenna proteins,photosynthesis,and plant hormone signal transduction path-ways.It was relatively found that sugar substances accumulated in RcGSTF11-OE Arabidopsis plants.In addition,the expression of RcGSTF11 can reduce the accumulation of ROS,increase the GSH/GSSG ratio,and help maintain the re-dox homeostasis in plants and reshape the transcription of downstream genes.In summary,Rc GSTF11 was found to have a dual regulatory effect of proximal interaction with RBCL to regulate photosynthetic carbon assimilation and distal regulation of redox homeostasis.