X射线辐照对北沙参幼苗生长及生理生化的影响OA

To study the effects of X-ray irradiation on the growth,physiology,and biochemistry of Glehniae Radix(Beishashen)seedlings,dry seeds were irradiated with different doses of X-rays(0,20,40,60,80,100,120,and 150 Gy)and subsequently planted for growth observation.Germination rate and seedling survival rate under different irradiation doses were recorded.Morphological indicators such as leaf number and root length,as well as physiological and bio-chemical parameters including chlorophyll,proline(Pro),and malondialdehyde(MDA)contents,were measured to sys-tematically analyze the effects of irradiation on Glehniae Radix seedlings.The results showed that the effects of X-ray irradiation on Glehniae Radix seedlings were dose-dependent.Low-dose irradiation(20 Gy)had minimal impact on cer-tain morphological and physiological indicators.Medium to high doses(40 Gy and above)generally exhibited inhibitory effects,which were particularly evident in terms of germination rate,survival rate,and root growth.High-dose irradiation(80 Gy and above)significantly suppressed seedling growth,leading to a sharp decline in both germination and survival rates.Based on a comprehensive evaluation of all indicators,the potential suitable dose range for X-ray irradiation-induced mutation breeding in Glehniae Radix is suggested to be 20-40 Gy.