不同微生物菌剂接种对盐碱地绿豆叶片生理代谢的调控效应OA

Soil salinization is a key abiotic stress restricting agricultural production,which seriously inhibits crop photosynthetic physiological metabolism and yield formation.The use of microbial agents to alleviate crop saline-alkali stress has become a research hotspot in the field of agricultural ecology.The new mung bean variety Zhonglv 27 was used as the material.Under the condition of saline-alkali land in the Xiliaohe Plain,no inoculation control(CK),rhizo-bium(R),arbuscular mycorrhizal fungi(AMF),rhizosphere growth-promoting bacteria(PGPR)single inoculation,and R+AMF,R+PGPR,R+AMF+PGPR and other compound inoculation treatments were set up to explore the effects of differ-ent microbial agents and combined inoculation on photosynthetic gas exchange parameters,sugar metabolite content and water use efficiency of mung bean at flowering-podding stages.The results showed that the inoculation of bacterial agent could significantly alleviate the inhibitory effect of saline-alkali stress on the physiological metabolism of mung bean leaves,and the effect of compound inoculation was better than that of single inoculation.Among them,the photo-synthetic gas exchange parameters such as net photosynthetic rate and stomatal conductance of leaves and the contents of soluble sugar(4.05 mg/g·FW),sucrose(4.09 mg/g·FW)and starch(2.40 mg/g·FW)in R+AMF+PGPR ternary com-pound inoculation treatment were significantly higher than those in other treatments(P＜0.05).The WUE of R+AMF+PGPR was the best(3.77 μmol CO2/mmol H2O),followed by R single inoculation treatment(3.69 μmol CO2/mmol H2O),both of which were significantly better than CK and most compound treatments.In summary,rhizobium-AMF-PGPR ternary compound inoculation can synergistically improve photosynthetic efficiency,enhance carbon assimilation capac-ity,optimize water use strategy,and effectively enhance the stress resistance of mung bean under saline-alkali stress.