普通小麦-野生二粒小麦远缘杂交后代穗发芽抗性鉴定和分子标记分析OA

Wild emmer wheat,a close relative of common wheat,has many excellent traits and resist-ance genes.Its hybridization with common wheat helps to cultivate innovative germplasm,expand the genetic base of wheat,and enhance its stress resilience.Using the whole spike germination method(SGR),28 new wheat lines derived from distant hybridization and backcrossing between wild emmer wheat and common wheat were evaluated for spike germination resistance,and the experimental ma-terials were genotyped using 4 functional molecular markers for pre-harvest sprouting resistance(PM19-A1,myb10D,Vp1B3,and Dorm-B1).The results showed that the relative spike germination in-dex of the 28 wheat progeny lines ranged from 0 to 1.09,with an average relative spike germination index of 0.38.Among them,six lines were highly resistant resistance,accounting for 21.43％;three lines were re-sistant,accounting for 10.71％;nine lines showed moderate resistance,accounting for 32.14％;two lines were susceptible,accounting for 7.14％;and eight lines were highly susceptible,accounting for 28.57％.By analyzing the alleles for pre-harvest sprouting resistance,the allelic combination PM19-A1a/Vp-1Bc/myb10-D1b/Dorm-B1a had the lowest average relative spike germination index of 0.13,while PM19-A1b/Vp-1 Ba/myb10-D1b/Dorm-B1a had the highest average relative spike ger-mination index of 0.70.Based on comprehensive phenotypic identification and molecular marker de-tection,six lines,namely MM-3-1,MM-3-5,MM-3-7,MM-3-0,MM-4(12-20)-2,and MM-4-8,were selected for prioritizing utilization as germplasm resources resistant to pre-harvest sprouting.